The first experiment I ran showed no difference between the wounded and the unwounded seaweeds. I decided to do the experiment again but only run it for 24 hours instead of 48, and I put the samples in the dark so that the amphipods would feed faster. I also cut off the tips of all the seaweed before I wounded them or not.
During the first experiment I was trying to see if the whole seaweed turned on chemical defenses to make itself not tasty to the herbivores; this time, I was seeing if the wounding would effect just a small part of the tip. Both times, however, there were not significant differences between the wounded and unwounded seaweeds. Next, I am going to run another experiment where I either wound or do not wound the tissue, but then I'm going to freeze the seaweeds and grind them up and feed them to the herbivores. It's possible that the seaweeds, when left alive and in water with the amphipods, stop putting out their chemical defenses after a short period of time. If I freeze the tissue right after I grind it, I'm hoping it will freeze the chemical defense the seaweed is putting off. I'll let you know the results I get!
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Updates from Sarah:
I'm still doing a lot of temperature assays. Because the seaweed that was frozen for up to 1 hour survived, I ran another cold assay experiment the next week. I put samples in the freezer for each of the following time periods: 1 hour, 2 hours, 3 hours, and 4 hours. All samples that were frozen for 2 or more hours were dead a week after the experiment. This week, I'll be running another cold assay to try and figure out the time point between 1 and 2 hours at which the seaweed reaches its limit. I'll be putting samples in the freezer for the following time periods: 45 minutes, 1 hour, 1 hr 15 min, 1 hr 30 min, 1 hr 45 min, and 2 hours. I'll also use a control group that won't go in the freezer at all. I've also been playing with an image analysis software called Image J. This software can help me measure the length of the seaweed tips that I use in the temperature assay experiments-the tips are so small that they are difficult to measure with a ruler. Image J can also help quantify color-I've been classifying the samples as dead/bleached, dying/partially bleached, or alive/no bleaching based on qualitative observations of color. However, this is a pretty subjective way to measure color- if a sample looks a bit lighter 1 week after the experiment to me, someone else could look at it and think it looks exactly the same. The blue and black vs. white and gold dress controversy is a good example of how color can be subjective! http://www.nytimes.com/interactive/2015/02/28/science/white-or-blue-dress.html?_r=0 Image J quantitatively measures the amount of red, green, and blue (RGB) in a selected piece of seaweed, so I'll be using this from now on in addition to my own observations. Erik and I have sent out one of the first manuscripts describing the invasive history of Gracilaria vermiculophylla to all of our co-authors! This is one step closer to getting it submitted for publication. It is the culmination of several years of work generating the data and several months of solid work analyzing all the data and crafting the story!
We've all also been really busy planning our first sampling trip to Japan which starts in just a few weeks! We are still working out protocols for all the sampling! I am hoping to get back in the lab to do a bit of genetic work before travel begins, but with several more manuscripts on my desk, I foresee some long days (and nights) in the lab/desk -Stacy |
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Patriots Point Naval and Maritime Museum
40 Patriots Point Rd.
Mt. Pleasant, SC 29464
40 Patriots Point Rd.
Mt. Pleasant, SC 29464